Assisted Laser Hatching

Assisted Layer Hatching

A ssisted layer hatching is the procedure done with the embryos when it is there in the laboratory itself. Prior to the time the embryo gets attached to the wall of the womb, there is need for it to hatch from its outer layer called as Zona pellucida . This procedure is carried out by making a hole in the zona pellucida layer by using laser or mechanical method to help the embryos to hatch, so as to increase the probability of conceiving and getting pregnant. 

Procedure Of Assisted Layer Hatching

Assisted layer hatching is the three step procedure carried out by the experts. The detailed procedure is as follows:

Step 1: On third day of embryo development, the weak acid in a fine glass pipette, a micro laser or a microtool to thin or cut a hole in the outer layer is used by the embryologist.

Step 2:  The embryo is washed to prevent further damage, if weak acid is put into use.

Step 3: Assisted hatching makes a hole in the zona pellucida layer around the embryo, the female is given antibiotics so as to prevent the infection.

Chances of conceiving with assisted hatching

It is the age of the woman which plays a role in deciding the success of the assisted layer hatching. The fertility of the females decreases as they get aged. The percentage of cycles that resulted in live birth are as follows:

 6% for women with the age of the 43-44

9% for women with the age of 40-42

5% for women with the age of 38-39

1% for women with the age of 35-37

2% for women with age under 35

Conditions under which Assisted Layer hatching is recommended


The assisted layer hatching is recommended for the patients which are any of these following conditions listed below:

Frozen Thaw embryos

History of failed IVF cycles

Excessive fragmentation of the existing embryos

Thick or abnormal shell

Age greater than 37 years

Patients with the elevated day 3 follicle stimulating hormone

Development of poor quality embryo

Risks associated with the assisted hatching

For the patient, there are no differences observed for the treatment for the embryo transfer with the IVF/ICSI procedure or the IVF/ICSI procedures with the layer hatching technique.

Factors associated with Assisted Layer Hatching

Various factors decide the assisted layer hatching results. There are two factors which determine the results. One of the factors is being the experience of the embryologist who is handling the patients. The other factor is being the characteristics of the patient.  For example, the age of the patient, if the age of the patient is above 38, the live rates are reduced. Controversy related to this is that although this is a beneficial procedure but still, some of the complications might occur. This can be the damage of the embryos during the micromanipulation technique which might lead to IVF failure. This causes the decrease in the boost of the live birth rates.